Chapter title |
Analysis of Terpenoid Indole Alkaloids, Carotenoids, Phytosterols, and NMR-Based Metabolomics for Catharanthus roseus Cell Suspension Cultures
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Chapter number | 31 |
Book title |
Plant Cell Culture Protocols
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8594-4_31 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8593-7, 978-1-4939-8594-4
|
Authors |
Mohd Zuwairi Saiman, Natali Rianika Mustafa, Robert Verpoorte, Saiman, Mohd Zuwairi, Mustafa, Natali Rianika, Verpoorte, Robert |
Abstract |
The plant Catharanthus roseus is a rich source of terpenoid indole alkaloids (TIA). Some of the TIA are important as antihypertensive (ajmalicine) and anticancer (vinblastine and vincristine) drugs. However, production of the latter is very low in the plant. Therefore, in vitro plant cell cultures have been considered as a potential supply of these chemicals or their precursors. Some monomeric alkaloids can be produced by plant cell cultures, but not on a level feasible for commercialization, despite extensive studies on this plant that deepened the understanding of the TIA biosynthesis and its regulation. In order to analyze the metabolites in C. roseus cell cultures, this chapter presents the method of TIA, carotenoids, and phytosterols analyses. Furthermore, an NMR-based metabolomics approach to study C. roseus cell culture is described. |
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Demographic breakdown
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Student > Master | 3 | 20% |
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Professor | 1 | 7% |
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Other | 0 | 0% |
Unknown | 6 | 40% |
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Other | 1 | 7% |
Unknown | 6 | 40% |