Chapter title |
Using Flow Cytometry Analysis in Plant Tissue Culture Derived Plants
|
---|---|
Chapter number | 22 |
Book title |
Plant Cell Culture Protocols
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8594-4_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8593-7, 978-1-4939-8594-4
|
Authors |
Rosa María Escobedo-Gracia-Medrano, Martha Josefa Burgos-Tan, José Roberto Ku-Cauich, Adriana Quiroz-Moreno, Escobedo-Gracia-Medrano, Rosa María, Burgos-Tan, Martha Josefa, Ku-Cauich, José Roberto, Quiroz-Moreno, Adriana |
Abstract |
Somaclonal variation (SC) in plants regenerated from tissue culture, via organogenesis or somatic embryogenesis, is frequently associated with abnormalities in the content of deoxyribonucleic acid (DNA), viz., aneuploidy and polyploidy. Flow cytometry (FCM) using the nucleic acid-specific fluorochrome propidium iodide has proven to be a rapid, simple, and reproducible technique for assessment of DNA content and ploidy variation occurring in plant tissue cultures. Here an outline of the sample preparation of suspension with intact nuclei by the two-step standard method, and FCM analysis of DNA ploidy stability in plants regenerated from embryogenic cell suspension (ECS) of banana Musa acuminata, AAA, cv. Grand Naine (Cavendish subgroup) using an internal standard is described. |
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