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miRNA Biogenesis

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Cover of 'miRNA Biogenesis'

Table of Contents

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    Book Overview
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    Chapter 1 High-Throughput Characterization of Primary microRNA Transcripts
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    Chapter 2 Identifying Pri-miRNA Transcription Start Sites
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    Chapter 3 Metabolic Pulse-Chase RNA Labeling for pri-miRNA Processing Dynamics
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    Chapter 4 In Vitro System for Coupling RNAP II Transcription to Primary microRNA Processing and a Three-Way System for RNAP II Transcription/Splicing/microRNA Processing
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    Chapter 5 Purification of Microprocessor-Associated Factors
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    Chapter 6 Inhibiting Pri-miRNA Processing with Target Site Blockers
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    Chapter 7 MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction
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    Chapter 8 MicroRNA Analysis Using Next-Generation Sequencing
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    Chapter 9 Identification of microRNA Precursor-Associated Proteins
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    Chapter 10 Analysis of 3′ End Modifications in microRNAs by High-Throughput Sequencing
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    Chapter 11 Uncovering the Stability of Mature miRNAs by 4-Thio-Uridine Metabolic Labeling
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    Chapter 12 Detection of microRNA-Target Interactions by Chimera PCR (ChimP)
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    Chapter 13 High-Quality Overlapping Paired-End Reads for the Detection of A-to-I Editing on Small RNA
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    Chapter 14 Targeting miRNA for Therapeutics Using a Micronome Based Method for Identification of miRNA-mRNA Pairs and Validation of Key Regulator miRNA
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    Chapter 15 Method for Detection of miRNAs in Non-Model Organisms with Unreported Database
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    Chapter 16 Detection and Verification of Mammalian Mirtrons by Northern Blotting
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    Chapter 17 Detecting Agotrons in Ago CLIPseq Data
Attention for Chapter 15: Method for Detection of miRNAs in Non-Model Organisms with Unreported Database
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Chapter title
Method for Detection of miRNAs in Non-Model Organisms with Unreported Database
Chapter number 15
Book title
miRNA Biogenesis
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8624-8_15
Pubmed ID
Book ISBNs
978-1-4939-8623-1, 978-1-4939-8624-8
Authors

Parveen Bansal, Ashish Kumar, Sudhir Chandna, Malika Arora, Renu Bansal, Bansal, Parveen, Kumar, Ashish, Chandna, Sudhir, Arora, Malika, Bansal, Renu

Abstract

Non-model organisms are studied very frequently, as a simple accessible and convenient system to investigate the role of miRNAs in particular aspect of biology or disease. However, the unavailability of the annotated genome and hence miRNA database of these non-model organisms pose a major constraint for using them more efficiently. Here, we describe a new method to identify miRNAs in non-model organisms without complex sequencing strategies and using miRNAs from close relative organisms as proxy/reference sequences.

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Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Librarian 1 25%
Student > Ph. D. Student 1 25%
Professor > Associate Professor 1 25%
Unknown 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 50%
Social Sciences 1 25%
Unknown 1 25%