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miRNA Biogenesis

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Cover of 'miRNA Biogenesis'

Table of Contents

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    Book Overview
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    Chapter 1 High-Throughput Characterization of Primary microRNA Transcripts
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    Chapter 2 Identifying Pri-miRNA Transcription Start Sites
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    Chapter 3 Metabolic Pulse-Chase RNA Labeling for pri-miRNA Processing Dynamics
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    Chapter 4 In Vitro System for Coupling RNAP II Transcription to Primary microRNA Processing and a Three-Way System for RNAP II Transcription/Splicing/microRNA Processing
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    Chapter 5 Purification of Microprocessor-Associated Factors
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    Chapter 6 Inhibiting Pri-miRNA Processing with Target Site Blockers
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    Chapter 7 MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction
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    Chapter 8 MicroRNA Analysis Using Next-Generation Sequencing
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    Chapter 9 Identification of microRNA Precursor-Associated Proteins
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    Chapter 10 Analysis of 3′ End Modifications in microRNAs by High-Throughput Sequencing
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    Chapter 11 Uncovering the Stability of Mature miRNAs by 4-Thio-Uridine Metabolic Labeling
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    Chapter 12 Detection of microRNA-Target Interactions by Chimera PCR (ChimP)
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    Chapter 13 High-Quality Overlapping Paired-End Reads for the Detection of A-to-I Editing on Small RNA
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    Chapter 14 Targeting miRNA for Therapeutics Using a Micronome Based Method for Identification of miRNA-mRNA Pairs and Validation of Key Regulator miRNA
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    Chapter 15 Method for Detection of miRNAs in Non-Model Organisms with Unreported Database
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    Chapter 16 Detection and Verification of Mammalian Mirtrons by Northern Blotting
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    Chapter 17 Detecting Agotrons in Ago CLIPseq Data
Attention for Chapter 7: MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction
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Chapter title
MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction
Chapter number 7
Book title
miRNA Biogenesis
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8624-8_7
Pubmed ID
Book ISBNs
978-1-4939-8623-1, 978-1-4939-8624-8
Authors

Marta Kotlarek, Anna Kubiak, Krystian Jażdżewski, Anna Wójcicka, Kotlarek, Marta, Kubiak, Anna, Jażdżewski, Krystian, Wójcicka, Anna

Abstract

The analysis of microRNA expression patterns provides new insights into numerous cellular processes and their aberrances in diseases. Despite its potential pitfalls, the quantitative real-time polymerase chain reaction (qPCR) is the most commonly used tool for microRNA profiling. The method requires extraction and quality analysis of RNA, which is further reverse transcribed using specific primers and used as a template in a qPCR reaction. All these elements have been addressed in this chapter.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 100%