Chapter title |
Bioelectronic Nose Using Olfactory Receptor-Embedded Nanodiscs
|
---|---|
Chapter number | 18 |
Book title |
Olfactory Receptors
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8609-5_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8608-8, 978-1-4939-8609-5
|
Authors |
Heehong Yang, Minju Lee, Daesan Kim, Seunghun Hong, Tai Hyun Park, Yang, Heehong, Lee, Minju, Kim, Daesan, Hong, Seunghun, Park, Tai Hyun |
Abstract |
Olfactory receptors (ORs) are the largest family of the G protein-coupled receptors (GPCRs), which are significantly involved in many human diseases and 40% of all drug targets. A platform containing stable and high-quality OR would be a powerful tool for the development of a practical biosensor that can be applied to various applications, such as the early diagnosis of diseases, assessment of food quality, and drug and fragrance development. Significant efforts have been made to develop the biosensor using GPCRs; nevertheless, they remain a challenge. This chapter describes an attractive methodology for the development of a stable bioelectronic nose using OR-embedded nanodiscs. The ORs were produced in Escherichia coli (E. coli), purified with column chromatography, reconstituted into nanodiscs and applied to a carbon nanotube-field effect transistor (CNT-FET) with floating electrodes. The nanodisc-based bioelectronic nose exhibits high-performance in terms of sensitivity, selectivity and stability. This strategy can be used as a practical method for the receptor-based sensing approach, which represents significant progress in nano-bio technology toward a practical biosensor. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 2 | 14% |
Student > Postgraduate | 2 | 14% |
Researcher | 2 | 14% |
Student > Ph. D. Student | 1 | 7% |
Unspecified | 1 | 7% |
Other | 1 | 7% |
Unknown | 5 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Veterinary Science and Veterinary Medicine | 1 | 7% |
Chemical Engineering | 1 | 7% |
Unspecified | 1 | 7% |
Agricultural and Biological Sciences | 1 | 7% |
Other | 2 | 14% |
Unknown | 6 | 43% |