Chapter title |
Fluorescence-Activated Cell Sorting of Olfactory Sensory Neuron Subpopulations
|
---|---|
Chapter number | 6 |
Book title |
Olfactory Receptors
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8609-5_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8608-8, 978-1-4939-8609-5
|
Authors |
Artur G. Leme Silva, Maíra H. Nagai, Bettina Malnic, Leme Silva, Artur G., Nagai, Maíra H., Malnic, Bettina |
Abstract |
The mouse olfactory epithelium is composed of a heterogeneous population of olfactory sensory neurons, where each neuron expresses one single type of odorant receptor gene, out of a repertoire of ~1000 different genes. Fluorescent-activated cell sorting (FACS) is a powerful technique, which can be used to isolate a cellular subpopulation from a heterogeneous tissue. The sorted neurons can then be used in gene expression studies, or analyzed for the presence of different DNA epigenetic modification marks. Here we describe a method to separate a subpopulation of olfactory sensory neurons expressing the odorant receptor Olfr17. In this method, the main olfactory epithelium from transgenic Olfr17-IRES-GFP mice is dissociated into single cells, followed by separation of the GFP positive cells by FACS. |
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