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DNA Replication

Overview of attention for book
Cover of 'DNA Replication'

Table of Contents

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    Book Overview
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    Chapter 1 A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
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    Chapter 2 Microscopy techniques to examine DNA replication in fission yeast.
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    Chapter 3 High-Resolution Analysis of Mammalian DNA Replication Units
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    Chapter 4 Analyzing the Dynamics of DNA Replication in Mammalian Cells Using DNA Combing
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    Chapter 5 Measuring DNA content by flow cytometry in fission yeast.
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    Chapter 6 Incorporation of thymidine analogs for studying replication kinetics in fission yeast.
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    Chapter 7 EdU Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast.
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    Chapter 8 Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection.
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    Chapter 9 Measuring ribonucleotide incorporation into DNA in vitro and in vivo.
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    Chapter 10 Detection and Sequencing of Okazaki Fragments in S. cerevisiae.
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    Chapter 11 ChIP-Seq to Analyze the Binding of Replication Proteins to Chromatin.
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    Chapter 12 Chromatin immunoprecipitation to detect DNA replication and repair factors.
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    Chapter 13 Molecular Genetic Methods to Study DNA Replication Protein Function in Haloferax volcanii, A Model Archaeal Organism.
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    Chapter 14 Single-Molecule Observation of Prokaryotic DNA Replication
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    Chapter 15 Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast.
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    Chapter 16 Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis
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    Chapter 17 Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes
Attention for Chapter 12: Chromatin immunoprecipitation to detect DNA replication and repair factors.
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Chapter title
Chromatin immunoprecipitation to detect DNA replication and repair factors.
Chapter number 12
Book title
DNA Replication
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2596-4_12
Pubmed ID
25916713
Book ISBNs
978-1-4939-2595-7, 978-1-4939-2596-4
Authors

Gadaleta, Mariana C, Iwasaki, Osamu, Noguchi, Chiaki, Noma, Ken-Ichi, Noguchi, Eishi, Mariana C. Gadaleta, Osamu Iwasaki, Chiaki Noguchi, Ken-Ichi Noma, Eishi Noguchi, Gadaleta, Mariana C.

Abstract

DNA replication is tightly coupled with DNA repair processes in order to preserve genomic integrity. During DNA replication, the replication fork encounters a variety of obstacles including DNA damage/adducts, secondary structures, and programmed fork-blocking sites, which are all difficult to replicate. The replication fork also collides with the transcription machinery, which shares the template DNA with the replisome complex. Under these conditions, replication forks stall, causing replication stress and/or fork collapse, ultimately leading to genomic instability. The mechanisms to overcome these replication problems remain elusive. Therefore, it is important to investigate how DNA repair and replication factors are recruited and coordinated at chromosomal regions that are difficult to replicate. In this chapter, we describe a chromatin immunoprecipitation method to locate proteins required for DNA repair during DNA replication in the fission yeast Schizosaccharomyces pombe. This method can also easily be adapted to study replisome components or chromatin-associated factors.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 36%
Student > Bachelor 2 18%
Student > Ph. D. Student 2 18%
Unknown 3 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 36%
Agricultural and Biological Sciences 4 36%
Unknown 3 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 April 2015.
All research outputs
#18,410,971
of 22,805,349 outputs
Outputs from Methods in molecular biology
#7,906
of 13,120 outputs
Outputs of similar age
#255,827
of 353,075 outputs
Outputs of similar age from Methods in molecular biology
#479
of 996 outputs
Altmetric has tracked 22,805,349 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,120 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,075 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.

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