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DNA Replication

Overview of attention for book
Cover of 'DNA Replication'

Table of Contents

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    Book Overview
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    Chapter 1 A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
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    Chapter 2 Microscopy techniques to examine DNA replication in fission yeast.
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    Chapter 3 High-Resolution Analysis of Mammalian DNA Replication Units
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    Chapter 4 Analyzing the Dynamics of DNA Replication in Mammalian Cells Using DNA Combing
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    Chapter 5 Measuring DNA content by flow cytometry in fission yeast.
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    Chapter 6 Incorporation of thymidine analogs for studying replication kinetics in fission yeast.
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    Chapter 7 EdU Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast.
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    Chapter 8 Determination of deoxyribonucleoside triphosphate concentrations in yeast cells by strong anion-exchange high-performance liquid chromatography coupled with ultraviolet detection.
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    Chapter 9 Measuring ribonucleotide incorporation into DNA in vitro and in vivo.
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    Chapter 10 Detection and Sequencing of Okazaki Fragments in S. cerevisiae.
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    Chapter 11 ChIP-Seq to Analyze the Binding of Replication Proteins to Chromatin.
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    Chapter 12 Chromatin immunoprecipitation to detect DNA replication and repair factors.
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    Chapter 13 Molecular Genetic Methods to Study DNA Replication Protein Function in Haloferax volcanii, A Model Archaeal Organism.
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    Chapter 14 Single-Molecule Observation of Prokaryotic DNA Replication
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    Chapter 15 Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast.
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    Chapter 16 Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis
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    Chapter 17 Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes
Attention for Chapter 15: Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast.
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Chapter title
Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast.
Chapter number 15
Book title
DNA Replication
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2596-4_15
Pubmed ID
25916716
Book ISBNs
978-1-4939-2595-7, 978-1-4939-2596-4
Authors

Mohebi, Saed, Lambert, Sarah A E, Carr, Antony M, Saed Mohebi, Sarah A. E. Lambert, Antony M. Carr, Lambert, Sarah A. E., Carr, Antony M.

Abstract

The study of how eukaryotic cells overcome problems associated with dysfunctional DNA replication forks is assisted by experimental systems that allow site-specific replication fork arrest. Here we provide protocols for the use of the fission yeast RTS1 replication fork barrier. The RTS1 barrier is a directional, or polar, replication fork barrier that evolved to ensure directional replication of the fission yeast mating-type locus. We have moved the 859 bp RTS1 sequence to another locus in the genome and demonstrated that it arrests replication forks in a dysfunctional confirmation and that replication is restarted within ~20 min by the action of homologous recombination. We describe here the barrier constructs currently available, the methods by which we regulate the activity of the barrier, how to synchronize cells for analysis of replication intermediates by 2D gel electrophoresis, and the use of a replication slippage assay to measure fork fidelity.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 43%
Student > Ph. D. Student 1 14%
Student > Master 1 14%
Unknown 2 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 43%
Agricultural and Biological Sciences 2 29%
Unknown 2 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 April 2015.
All research outputs
#15,683,389
of 23,305,591 outputs
Outputs from Methods in molecular biology
#5,496
of 13,332 outputs
Outputs of similar age
#211,780
of 355,651 outputs
Outputs of similar age from Methods in molecular biology
#354
of 1,004 outputs
Altmetric has tracked 23,305,591 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,332 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 355,651 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,004 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.

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