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X Demographics
Mendeley readers
Attention Score in Context
| Chapter title |
Measuring DNA content by flow cytometry in fission yeast.
|
|---|---|
| Chapter number | 5 |
| Book title |
DNA Replication
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2596-4_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2595-7, 978-1-4939-2596-4
|
| Authors |
Sabatinos, Sarah A, Forsburg, Susan L, Sarah A. Sabatinos, Susan L. Forsburg, Sabatinos, Sarah A., Forsburg, Susan L. |
| Abstract |
Flow cytometry is an essential tool to monitor DNA content and determine cell cycle distribution. Its utility in fission yeast reflects the ease of sample preparation, the stochiometric binding of the most popular DNA dyes (propidium iodide and Sytox Green), and ability to monitor cell size. However, the study of DNA replication with multicolour flow analysis has lagged behind its use in mammalian cells. We present basic and advanced protocols for analysis of DNA replication in fission yeast by flow cytometry including whole cell, nuclear "ghosts," two-color imaging with BrdU, and estimates of DNA synthesis using EdU. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 4% |
| Unknown | 26 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 22% |
| Student > Master | 5 | 19% |
| Student > Bachelor | 4 | 15% |
| Researcher | 4 | 15% |
| Professor > Associate Professor | 2 | 7% |
| Other | 3 | 11% |
| Unknown | 3 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 41% |
| Agricultural and Biological Sciences | 10 | 37% |
| Unspecified | 1 | 4% |
| Chemistry | 1 | 4% |
| Medicine and Dentistry | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 3 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 April 2015.
All research outputs
#20,273,512
of 22,805,349 outputs
Outputs from Methods in molecular biology
#9,905
of 13,120 outputs
Outputs of similar age
#295,802
of 353,075 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
Altmetric has tracked 22,805,349 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,120 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,075 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.