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Mendeley readers
Chapter title |
Photoconvertible Fluorescent Protein-Based Live Imaging of Mitochondrial Fusion
|
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Chapter number | 18 |
Book title |
Cell Fusion
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Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2703-6_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2702-9, 978-1-4939-2703-6
|
Authors |
Geoffrey C. Owens, David B. Edelman, Owens, Geoffrey C., Edelman, David B. |
Abstract |
Mitochondria are highly dynamic organelles that undergo fusion and fission on a relatively fast time scale. Here, a straightforward method is described for capturing mitochondrial fusion events in real time using a photoconvertible fluorescent protein and a far-field fluorescence microscope equipped with appropriate image acquisition and analysis software. The Kaede photoconvertible fluorescent protein is tagged with a mitochondrial targeting sequence and delivered to primary neurons by lentiviral transduction, which ensures efficient low copy number transgene insertion, as well as stable transgene expression. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 33% |
Student > Ph. D. Student | 2 | 22% |
Student > Doctoral Student | 1 | 11% |
Unknown | 3 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 3 | 33% |
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Unknown | 4 | 44% |