| Chapter title |
FRET in the Analysis of In Vitro Cell–Cell Fusion by Flow Cytometry
|
|---|---|
| Chapter number | 16 |
| Book title |
Cell Fusion
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2703-6_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2702-9, 978-1-4939-2703-6
|
| Authors |
Guillermo Gómez-Icazbalceta, Mirna Berenice Ruiz-Rivera, Edmundo Lamoyi, Leonor Huerta, Gómez-Icazbalceta, Guillermo, Ruiz-Rivera, Mirna Berenice, Lamoyi, Edmundo, Huerta, Leonor |
| Abstract |
Cell-cell fusion is a frequent event in nature leading to modification of cell fate. In this chapter, we describe a flow cytometric procedure for the quantitative assessment of in vitro cell-cell fusion events that allows the discrimination of fused from aggregated cells. The assay is based on the differential labeling of fusion partners with lipophilic fluorescent probes DiI (red) and DiO (green). Double fluorescent fused cells can be detected after coculturing by means of a flow cytometer equipped with a 488 nm laser. Fusion events can be distinguished from cell aggregates by the enhancement of the DiI red fluorescence intensity due to resonance energy transfer between the two probes occurring in the fused but not in the aggregated cell population. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 27% |
| Researcher | 2 | 18% |
| Student > Bachelor | 1 | 9% |
| Unspecified | 1 | 9% |
| Student > Ph. D. Student | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 2 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Agricultural and Biological Sciences | 2 | 18% |
| Unspecified | 1 | 9% |
| Immunology and Microbiology | 1 | 9% |
| Psychology | 1 | 9% |
| Other | 2 | 18% |
| Unknown | 2 | 18% |