Biotechnology of Plant Secondary Metabolism
Springer New York
Chapter title |
Protoplast Transformation as a Plant-Transferable Transient Expression System
|
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Chapter number | 13 |
Book title |
Biotechnology of Plant Secondary Metabolism
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3393-8_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3391-4, 978-1-4939-3393-8
|
Authors |
Patrícia Duarte, Diana Ribeiro, Inês Carqueijeiro, Sara Bettencourt, Mariana Sottomayor, Duarte, Patrícia, Ribeiro, Diana, Carqueijeiro, Inês, Bettencourt, Sara, Sottomayor, Mariana |
Abstract |
The direct uptake of DNA by naked plant cells (protoplasts) provides an expression system of exception for the quickly growing research in non-model plants, fuelled by the power of next-generation sequencing to identify novel candidate genes. Here, we describe a simple and effective method for isolation and transformation of protoplasts, and illustrate its application to several plant materials. |
Country | Count | As % |
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Unknown | 20 | 100% |
Readers by professional status | Count | As % |
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Researcher | 4 | 20% |
Student > Ph. D. Student | 3 | 15% |
Student > Master | 2 | 10% |
Student > Doctoral Student | 1 | 5% |
Student > Bachelor | 1 | 5% |
Other | 3 | 15% |
Unknown | 6 | 30% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 7 | 35% |
Biochemistry, Genetics and Molecular Biology | 5 | 25% |
Environmental Science | 1 | 5% |
Engineering | 1 | 5% |
Unknown | 6 | 30% |