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Mendeley readers
Chapter title |
Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
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Chapter number | 9 |
Book title |
Biotechnology of Plant Secondary Metabolism
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3393-8_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3391-4, 978-1-4939-3393-8
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Authors |
Fernanda de Costa, Carla J. S. Barber, Pareshkumar T. Pujara, Darwin W. Reed, Patrick S. Covello, de Costa, Fernanda, Barber, Carla J. S., Pujara, Pareshkumar T., Reed, Darwin W., Covello, Patrick S. |
Abstract |
Short peptide tags genetically fused to recombinant proteins have been widely used to facilitate detection or purification without the need to develop specific procedures. In general, an ideal affinity tag would allow the efficient purification of tagged proteins in high yield, without affecting its function. Here, we describe the purification steps to purify a recombinant polyhistidine-tagged glucosyltransferase from Centella asiatica using immobilized metal affinity chromatography. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 6 | 50% |
Lecturer | 1 | 8% |
Student > Postgraduate | 1 | 8% |
Student > Master | 1 | 8% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 58% |
Agricultural and Biological Sciences | 2 | 17% |
Unknown | 3 | 25% |