Chapter title |
A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
|
---|---|
Chapter number | 10 |
Book title |
Biotechnology of Plant Secondary Metabolism
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3393-8_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3391-4, 978-1-4939-3393-8
|
Authors |
Morten Egevang Jørgensen, Hussam Hassan Nour-Eldin, Barbara Ann Halkier, Jørgensen, Morten Egevang, Nour-Eldin, Hussam Hassan, Halkier, Barbara Ann |
Abstract |
Oocytes of the African clawed frog, Xenopus laevis, are often used for expression and biochemical characterization of transporter proteins as the oocytes are particularly suitable for uptake assays and electrophysiological recordings. Assessment of the expression level of expressed transporters at the individual oocyte level is often desirable when comparing properties of wild type and mutant transporters. However, a large content of yolk platelets in the oocyte cytoplasm makes this a challenging task. Here we report a method for fast and easy, semiquantitative Western blot analysis of proteins heterologously expressed in Xenopus oocytes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 2 | 17% |
Student > Bachelor | 2 | 17% |
Student > Ph. D. Student | 1 | 8% |
Student > Master | 1 | 8% |
Researcher | 1 | 8% |
Other | 2 | 17% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 25% |
Environmental Science | 1 | 8% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Economics, Econometrics and Finance | 1 | 8% |
Physics and Astronomy | 1 | 8% |
Other | 1 | 8% |
Unknown | 4 | 33% |