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Mendeley readers
Chapter title |
Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group
|
---|---|
Chapter number | 9 |
Book title |
Cytochrome P450 Protocols
|
Published in |
Methods in molecular biology, January 2013
|
DOI | 10.1007/978-1-62703-321-3_9 |
Pubmed ID | |
Book ISBNs |
978-1-62703-320-6, 978-1-62703-321-3
|
Authors |
Wesley E. Straub, Clinton R. Nishida, Paul R. Ortiz de Montellano, Straub, Wesley E., Nishida, Clinton R., Montellano, Paul R. Ortiz de |
Abstract |
Unlike many hemoproteins, the prosthetic heme group of most cytochrome P450 enzymes cannot be extracted and replaced by modified heme groups. Here, we describe a procedure for generating a cytochrome P450 enzyme (CYP119) with cobalt protoporphyrin IX as its prosthetic group. This is achieved by expressing the protein in Escherichia coli in iron-limited medium and adding cobalt to the medium at the moment that inducible protein expression is initiated. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 13% |
Unknown | 7 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 50% |
Researcher | 3 | 38% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 38% |
Chemistry | 3 | 38% |
Computer Science | 1 | 13% |
Unknown | 1 | 13% |