Cytochrome P450 Protocols

Chapter 2 Simultaneous Determination of Multiple CYP Inhibition Constants using a Cocktail-Probe Approach

Chapter 3 High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening

Chapter 4 The Synthesis, Characterization, and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites

Chapter 5 High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators

Chapter 6 Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches

Chapter 7 Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium tuberculosis.

Chapter 8 An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins

Chapter 9 Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group

Chapter 10 Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes

Chapter 11 Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles

Chapter 12 Fluorescence-Based Screening of Cytochrome P450 Activities in Intact Cells

Chapter 13 Screening for Cytochrome P450 Reactivity with a Reporter Enzyme

Chapter 14 High-Throughput Fluorescence Assay of Cytochrome P450 3A4.

Chapter 15 Targeted Protein Capture for Analysis of Electrophile-Protein Adducts

Chapter 16 DNA Shuffling of Cytochrome P450 Enzymes

Chapter 17 Measurement of P450 Difference Spectra Using Intact Cells

Chapter 18 DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production

Chapter 19 P450 Oxidoreductase: Genotyping, Expression, Purification of Recombinant Protein, and Activity Assessments of Wild-Type and Mutant Protein

Chapter 20 LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450

Chapter 21 Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-Allele) Nomenclature Database

Chapter 22 Simultaneous In Vivo Phenotyping of CYP Enzymes

Chapter 23 Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase I Footprinting

Chapter 24 Isolation of Mouse Hepatocytes

Chapter 25 Highly Efficient SiRNA and Gene Transfer into Hepatocyte-Like HepaRG Cells and Primary Human Hepatocytes: New Means for Drug Metabolism and Toxicity Studies