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Cytochrome P450 Protocols

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Cover of 'Cytochrome P450 Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Bioluminescent Assays for Cytochrome P450 Enzymes
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    Chapter 2 Simultaneous Determination of Multiple CYP Inhibition Constants using a Cocktail-Probe Approach
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    Chapter 3 High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening
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    Chapter 4 The Synthesis, Characterization, and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites
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    Chapter 5 High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators
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    Chapter 6 Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches
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    Chapter 7 Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium tuberculosis.
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    Chapter 8 An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins
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    Chapter 9 Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group
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    Chapter 10 Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes
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    Chapter 11 Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles
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    Chapter 12 Fluorescence-Based Screening of Cytochrome P450 Activities in Intact Cells
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    Chapter 13 Screening for Cytochrome P450 Reactivity with a Reporter Enzyme
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    Chapter 14 High-Throughput Fluorescence Assay of Cytochrome P450 3A4.
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    Chapter 15 Targeted Protein Capture for Analysis of Electrophile-Protein Adducts
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    Chapter 16 DNA Shuffling of Cytochrome P450 Enzymes
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    Chapter 17 Measurement of P450 Difference Spectra Using Intact Cells
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    Chapter 18 DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production
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    Chapter 19 P450 Oxidoreductase: Genotyping, Expression, Purification of Recombinant Protein, and Activity Assessments of Wild-Type and Mutant Protein
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    Chapter 20 LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450
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    Chapter 21 Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-Allele) Nomenclature Database
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    Chapter 22 Simultaneous In Vivo Phenotyping of CYP Enzymes
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    Chapter 23 Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase I Footprinting
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    Chapter 24 Isolation of Mouse Hepatocytes
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    Chapter 25 Highly Efficient SiRNA and Gene Transfer into Hepatocyte-Like HepaRG Cells and Primary Human Hepatocytes: New Means for Drug Metabolism and Toxicity Studies
Attention for Chapter 4: The Synthesis, Characterization, and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites
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Chapter title
The Synthesis, Characterization, and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites
Chapter number 4
Book title
Cytochrome P450 Protocols
Published in
Methods in molecular biology, January 2013
DOI 10.1007/978-1-62703-321-3_4
Pubmed ID
Book ISBNs
978-1-62703-320-6, 978-1-62703-321-3
Authors

Christopher McCullough, Phani Kumar Pullela, Sang-Choul Im, Lucy Waskell, Daniel Sem, McCullough, Christopher, Pullela, Phani Kumar, Im, Sang-Choul, Waskell, Lucy, Sem, Daniel

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 25%
Student > Ph. D. Student 1 25%
Researcher 1 25%
Student > Master 1 25%
Readers by discipline Count As %
Chemistry 2 50%
Pharmacology, Toxicology and Pharmaceutical Science 1 25%
Decision Sciences 1 25%
Biochemistry, Genetics and Molecular Biology 1 25%