Genotyping
Springer New York
Chapter title |
Genotyping DNA Variants with High-Resolution Melting Analysis
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Chapter number | 2 |
Book title |
Genotyping
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6442-0_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6440-6, 978-1-4939-6442-0
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Authors |
Rolf H. A. M. Vossen, Vossen, Rolf H. A. M. |
Abstract |
High-resolution melting analysis (HRMA) is a simple, quick, and effective method to scan and screen PCR amplicons for sequence variants. HRMA is a nondestructive closed tube assay; after PCR, DNA melting can directly be performed on the amplified samples without any purification or separation steps. For single SNP genotyping, HRMA is an attractive alternative to Sanger sequencing, restriction enzyme analysis, and hydrolysis probes. |
Country | Count | As % |
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Unknown | 20 | 100% |
Readers by professional status | Count | As % |
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Researcher | 3 | 15% |
Student > Ph. D. Student | 3 | 15% |
Student > Bachelor | 2 | 10% |
Student > Master | 2 | 10% |
Unspecified | 1 | 5% |
Other | 3 | 15% |
Unknown | 6 | 30% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 30% |
Agricultural and Biological Sciences | 3 | 15% |
Unspecified | 1 | 5% |
Veterinary Science and Veterinary Medicine | 1 | 5% |
Unknown | 9 | 45% |