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Genotyping

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Cover of 'Genotyping'

Table of Contents

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    Book Overview
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    Chapter 1 Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems
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    Chapter 2 Genotyping DNA Variants with High-Resolution Melting Analysis
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    Chapter 3 High-Throughput Genotyping with TaqMan Allelic Discrimination and Allele-Specific Genotyping Assays
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    Chapter 4 In Situ Single-Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification
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    Chapter 5 The MassARRAY® System for Targeted SNP Genotyping
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    Chapter 6 Genotyping
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    Chapter 7 Genotyping
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    Chapter 8 Analysis of Copy Number Variation Using the Paralogue Ratio Test (PRT)
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    Chapter 9 Genotyping Multiallelic Copy Number Variation with Multiplex Ligation-Dependent Probe Amplification (MLPA)
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    Chapter 10 Analysis of Multiallelic CNVs by Emulsion Haplotype Fusion PCR
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    Chapter 11 Quantitative DNA Analysis Using Droplet Digital PCR
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    Chapter 12 Full-Length Mitochondrial-DNA Sequencing on the PacBio RSII
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    Chapter 13 Targeted Locus Amplification and Next-Generation Sequencing
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    Chapter 14 Genotyping
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    Chapter 15 Rapid SNP Detection and Genotyping of Bacterial Pathogens by Pyrosequencing
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    Chapter 16 Methods for Genotyping-by-Sequencing
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    Chapter 17 Describing Sequence Variants Using HGVS Nomenclature
Attention for Chapter 5: The MassARRAY® System for Targeted SNP Genotyping
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Chapter title
The MassARRAY® System for Targeted SNP Genotyping
Chapter number 5
Book title
Genotyping
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6442-0_5
Pubmed ID
Book ISBNs
978-1-4939-6440-6, 978-1-4939-6442-0
Authors

Justine A. Ellis, Benjamin Ong, Ellis, Justine A., Ong, Benjamin

Abstract

Research to understand the genetic basis of disease, particularly complex disease, regularly involves single nucleotide polymorphism (SNP) genotyping. The use of genome-wide SNP genotyping arrays has become increasingly more commonplace for gene discovery. However, smaller-scale genotyping platforms capable of efficiently genotyping tens to hundreds of SNPs are still crucial for many aspects of this work, including replication of associations. The Agena Bioscience MassARRAY System is one such platform. Here, we provide a guide to using the MassARRAY System, from assay design, through mass spectrometry, to generation of genotype data.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 47 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 17%
Student > Master 6 13%
Student > Ph. D. Student 4 9%
Student > Bachelor 3 6%
Student > Doctoral Student 2 4%
Other 7 15%
Unknown 17 36%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 26%
Agricultural and Biological Sciences 4 9%
Medicine and Dentistry 4 9%
Pharmacology, Toxicology and Pharmaceutical Science 3 6%
Computer Science 2 4%
Other 4 9%
Unknown 18 38%