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Mendeley readers
| Chapter title |
Quantitative DNA Analysis Using Droplet Digital PCR
|
|---|---|
| Chapter number | 11 |
| Book title |
Genotyping
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6442-0_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6440-6, 978-1-4939-6442-0
|
| Authors |
Rolf H. A. M Vossen, Stefan J. White, Vossen, Rolf H. A. M, White, Stefan J. |
| Abstract |
Droplet digital PCR (ddPCR) is based on the isolated amplification of thousands of individual DNA molecules simultaneously, with each molecule compartmentalized in a droplet. The presence of amplified product in each droplet is indicated by a fluorescent signal, and the proportion of positive droplets allows the precise quantification of a given sequence. In this chapter we briefly outline the basis of ddPCR, and describe two different applications using the Bio-Rad QX200 system: genotyping copy number variation and quantification of Illumina sequencing libraries. |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 20% |
| Student > Ph. D. Student | 4 | 16% |
| Student > Bachelor | 2 | 8% |
| Other | 1 | 4% |
| Librarian | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 9 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 32% |
| Agricultural and Biological Sciences | 3 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Arts and Humanities | 1 | 4% |
| Economics, Econometrics and Finance | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 10 | 40% |