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Quantitative Real-Time PCR

Overview of attention for book
Cover of 'Quantitative Real-Time PCR'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Twenty Years of qPCR: A Mature Technology?
  3. Altmetric Badge
    Chapter 2 Minimum Information Necessary for Quantitative Real-Time PCR Experiments
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    Chapter 3 Selection of Reliable Reference Genes for RT-qPCR Analysis.
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    Chapter 4 Introduction to Digital PCR
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    Chapter 5 mRNA and microRNA Purity and Integrity: The Key to Success in Expression Profiling
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    Chapter 6 Quantitative Real-Time PCR
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    Chapter 7 Absolute Quantification of Viral DNA: The Quest for Perfection
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    Chapter 8 A Multiplex Real-Time PCR-Platform Integrated into Automated Extraction Method for the Rapid Detection and Measurement of Oncogenic HPV Type-Specific Viral DNA Load from Cervical Samples
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    Chapter 9 Real-Time PCR Detection of Mycoplasma pneumoniae in the Diagnosis of Community-Acquired Pneumonia.
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    Chapter 10 A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP Condition
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    Chapter 11 Real-time Quantification Assay to Monitor BCR-ABL1 Transcripts in Chronic Myeloid Leukemia
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    Chapter 12 A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCR
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    Chapter 13 Circulating Cell-Free DNA in Cancer
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    Chapter 14 Gene Expression Analysis by qPCR in Clinical Kidney Transplantation
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    Chapter 15 Posttranscriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA and MicroRNA Data
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    Chapter 16 Quantitative Real-Time PCR
  18. Altmetric Badge
    Chapter 17 Quantitative Real-Time PCR
Attention for Chapter 2: Minimum Information Necessary for Quantitative Real-Time PCR Experiments
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (82nd percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

Mentioned by

1 blog
1 tweeter


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Chapter title
Minimum Information Necessary for Quantitative Real-Time PCR Experiments
Chapter number 2
Book title
Quantitative Real-Time PCR
Published in
Methods in molecular biology, April 2014
DOI 10.1007/978-1-4939-0733-5_2
Pubmed ID
Book ISBNs
978-1-4939-0732-8, 978-1-4939-0733-5

Johnson G, Nour AA, Nolan T, Huggett J, Bustin S, Gemma Johnson, Afif Abdel Nour, Tania Nolan, Jim Huggett, Stephen Bustin, Johnson, Gemma, Nour, Afif Abdel, Nolan, Tania, Huggett, Jim, Bustin, Stephen


The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines were published in 2009 with the twin aims of providing a blueprint for good real-time quantitative polymerase chain reaction (qPCR) assay design and encouraging the comprehensive reporting of qPCR protocols. It had become increasingly clear that variable pre-assay conditions, poor assay design, and incorrect data analysis were leading to the routine publication of data that were often inconsistent, inaccurate, and wrong. The problem was exacerbated by a lack of transparency of reporting, with the details of technical information inadequate for the purpose of assessing the validity of published qPCR data. This had, and continues to have serious implications for basic research, reducing the potential for translating findings into valuable applications and potentially devastating consequences for clinical practice. Today, the rationale underlying the MIQE guidelines has become widely accepted, with more than 2,200 citations by March 2014 and editorials in Nature and related publications acknowledging the enormity of the problem. However, the problem we now face is rather serious: thousands of publications that report suspect data are populating and corrupting the peer-reviewed scientific literature. It will be some time before the many contradictions apparent in every area of the life sciences are corrected.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 101 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Indonesia 1 <1%
Chile 1 <1%
Austria 1 <1%
Brazil 1 <1%
Mexico 1 <1%
Russia 1 <1%
United States 1 <1%
Unknown 94 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 19 19%
Student > Bachelor 15 15%
Student > Ph. D. Student 15 15%
Student > Master 12 12%
Student > Doctoral Student 8 8%
Other 15 15%
Unknown 17 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 33%
Biochemistry, Genetics and Molecular Biology 21 21%
Medicine and Dentistry 6 6%
Chemistry 5 5%
Immunology and Microbiology 4 4%
Other 11 11%
Unknown 21 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 September 2014.
All research outputs
of 22,754,104 outputs
Outputs from Methods in molecular biology
of 13,089 outputs
Outputs of similar age
of 226,726 outputs
Outputs of similar age from Methods in molecular biology
of 149 outputs
Altmetric has tracked 22,754,104 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 226,726 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 149 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.