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Quantitative Real-Time PCR

Overview of attention for book
Cover of 'Quantitative Real-Time PCR'

Table of Contents

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    Book Overview
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    Chapter 1 Twenty Years of qPCR: A Mature Technology?
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    Chapter 2 Minimum Information Necessary for Quantitative Real-Time PCR Experiments
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    Chapter 3 Selection of Reliable Reference Genes for RT-qPCR Analysis.
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    Chapter 4 Introduction to Digital PCR
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    Chapter 5 mRNA and microRNA Purity and Integrity: The Key to Success in Expression Profiling
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    Chapter 6 Quantitative Real-Time PCR
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    Chapter 7 Absolute Quantification of Viral DNA: The Quest for Perfection
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    Chapter 8 A Multiplex Real-Time PCR-Platform Integrated into Automated Extraction Method for the Rapid Detection and Measurement of Oncogenic HPV Type-Specific Viral DNA Load from Cervical Samples
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    Chapter 9 Real-Time PCR Detection of Mycoplasma pneumoniae in the Diagnosis of Community-Acquired Pneumonia.
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    Chapter 10 A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP Condition
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    Chapter 11 Real-time Quantification Assay to Monitor BCR-ABL1 Transcripts in Chronic Myeloid Leukemia
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    Chapter 12 A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCR
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    Chapter 13 Circulating Cell-Free DNA in Cancer
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    Chapter 14 Gene Expression Analysis by qPCR in Clinical Kidney Transplantation
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    Chapter 15 Posttranscriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA and MicroRNA Data
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    Chapter 16 Quantitative Real-Time PCR
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    Chapter 17 Quantitative Real-Time PCR
Attention for Chapter 9: Real-Time PCR Detection of Mycoplasma pneumoniae in the Diagnosis of Community-Acquired Pneumonia.
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age and source (56th percentile)

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Chapter title
Real-Time PCR Detection of Mycoplasma pneumoniae in the Diagnosis of Community-Acquired Pneumonia.
Chapter number 9
Book title
Quantitative Real-Time PCR
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-0733-5_9
Pubmed ID
Book ISBNs
978-1-4939-0732-8, 978-1-4939-0733-5
Authors

Eddi Di Marco, Marco, Eddi Di

Abstract

Polymerase chain reaction is a useful technique in microbial diagnostics to detect and quantify DNA or RNA of low abundance. Bacterial and viral nucleic acid can be amplified by PCR upon clinical sample extraction using specific primers for classical qualitative PCR and primers and probes for real-time PCR. Here we describe the Scorpion-probe real-time PCR-based assay that offers thermodynamic advantages due to its kinetic reaction and provides faster performances compared to a classical double-labeled probe-based assays.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 13%
Unknown 7 88%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 25%
Student > Master 1 13%
Student > Bachelor 1 13%
Other 1 13%
Professor 1 13%
Other 1 13%
Unknown 1 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 25%
Agricultural and Biological Sciences 2 25%
Immunology and Microbiology 2 25%
Medicine and Dentistry 2 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 December 2014.
All research outputs
#15,300,431
of 22,755,127 outputs
Outputs from Methods in molecular biology
#5,313
of 13,089 outputs
Outputs of similar age
#190,008
of 305,246 outputs
Outputs of similar age from Methods in molecular biology
#200
of 597 outputs
Altmetric has tracked 22,755,127 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,246 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 597 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.