Chapter title |
Cell Imaging Techniques
|
---|---|
Chapter number | 6 |
Book title |
Cell Imaging Techniques
|
Published in |
Methods in molecular biology, January 2006
|
DOI | 10.1007/978-1-59259-993-6_6 |
Pubmed ID | |
Book ISBNs |
978-1-58829-157-8, 978-1-59259-993-6
|
Authors |
Douglas J. Taatjes, Brooke T. Mossman, Marilyn P. Wadsworth, Burton E. Sobel, David J. Schneider, Wendy Tra, Hans Hirtum, Wadsworth, Marilyn P, Sobel, Burton E, Schneider, David J, Tra, Wendy, van Hirtum, Hans, Taatjes, Douglas J, Hans van Hirtum, Wadsworth, Marilyn P., Sobel, Burton E., Schneider, David J., Hirtum, Hans van, Taatjes, Douglas J. |
Abstract |
Comparative quantitation has become an increasingly desirable tool in determining compositional differences of aortic plaque lesion in transgenically altered mice. To this end, methodology has been developed to identify lipid, cellularity, collagen, and elastin components using traditional bright-field microscopy, fluorescence, and polarized light microscopy, employing both confocal and wide-field imaging systems. Subsequent imaging processing and analysis on the digitally captured images reveals differences in compositional components as influenced by diet, age and gender. This method can be expanded to employ a rich variety of histochemical and immunohistochemical staining protocols. |
Mendeley readers
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Demographic breakdown
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