Chapter title |
Cell Imaging Techniques
|
---|---|
Chapter number | 11 |
Book title |
Cell Imaging Techniques
|
Published in |
Methods in molecular biology, January 2006
|
DOI | 10.1007/978-1-59259-993-6_11 |
Pubmed ID | |
Book ISBNs |
978-1-58829-157-8, 978-1-59259-993-6
|
Authors |
Manning, Christopher B, Mossman, Brooke T, Taatjes, Douglas J, Douglas J. Taatjes, Brooke T. Mossman, Christopher B. Manning, Manning, Christopher B., Mossman, Brooke T., Taatjes, Douglas J. |
Abstract |
Laser capture microdissection (LCM) enables the removal of discrete microstructures or cell types from properly prepared histological sections. Extraction of RNA from microdissected tissue followed by quantitative reverse transcriptase-polymerase chain (QRT-PCR) reaction permits the analysis of cell-type or microstructure-specific gene expression changes that occur in response to various stimuli in the environment. In our lab, the combination of LCM and QRT-PCR has proven very useful in the determination of the in vivo gene expression changes that occur in bronchiolar epithelium in response to inhalation of crocidolite asbestos. A detailed description of the preparation of cDNA from bronchiolar epithelial cells obtained by LCM is described in this work. |
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