Chapter title |
Quantification and Localization of S -Nitrosothiols (SNOs) in Higher Plants
|
---|---|
Chapter number | 12 |
Book title |
Plant Nitric Oxide
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3600-7_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3598-7, 978-1-4939-3600-7
|
Authors |
Juan B. Barroso, Raquel Valderrama, Alfonso Carreras, Mounira Chaki, Juan C. Begara-Morales, Beatriz Sánchez-Calvo, Francisco J. Corpas, Barroso, Juan B., Valderrama, Raquel, Carreras, Alfonso, Chaki, Mounira, Begara-Morales, Juan C., Sánchez-Calvo, Beatriz, Corpas, Francisco J. |
Abstract |
S-nitrosothiols (SNOs) are a family of molecules produced by the reaction of nitric oxide (NO) with -SH thiol groups present in the cysteine residues of proteins and peptides caused by a posttranslational modification (PTM) known as S-nitrosylation (strictly speaking S-nitrosation) that can affect the cellular function of proteins. These molecules are a relatively more stable form of NO and consequently can act as a major intracellular NO reservoir and, in some cases, as a long-distance NO signal. Additionally, SNOs can be transferred between small peptides and protein thiol groups through S-transnitrosylation mechanisms. Thus, detection and cellular localization of SNOs in plant cells can be useful tools to determine how these molecules are modulated under physiological and adverse conditions and to determine their importance as a mechanism for regulating different biochemical pathways. Using a highly sensitive chemiluminescence ozone technique and a specific fluorescence probe (Alexa Fluor 488 Hg-link phenylmercury), the methods described in this chapter enable us to determine SNOs in an nM range as well as their cellular distribution in the tissues of different plant species. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 31% |
Student > Master | 2 | 15% |
Lecturer > Senior Lecturer | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Student > Doctoral Student | 1 | 8% |
Other | 2 | 15% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 31% |
Agricultural and Biological Sciences | 2 | 15% |
Earth and Planetary Sciences | 1 | 8% |
Chemistry | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Other | 0 | 0% |
Unknown | 4 | 31% |