Chapter title |
Methods to Detect Nitric Oxide in Plants: Are DAFs Really Measuring NO?
|
---|---|
Chapter number | 6 |
Book title |
Plant Nitric Oxide
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3600-7_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3598-7, 978-1-4939-3600-7
|
Authors |
Stefan Ruemer, Markus Krischke, Agnes Fekete, Maria Lesch, Marin J. Mueller, Werner M. Kaiser, Ruemer, Stefan, Krischke, Markus, Fekete, Agnes, Lesch, Maria, Mueller, Marin J., Kaiser, Werner M. |
Abstract |
Nitric oxide, a gaseous radical molecule, appears involved in many reactions in all living organisms. Fluorescent dyes like DAF-2 and related compounds are still widely used to monitor NO production inside or outside cells, although doubts about their specificity have recently been raised. We present evidence that DAF dyes do not only react with nitric oxide but also with peroxidase enzyme and hydrogen peroxide. Both are secreted in the case of elicitation of tobacco suspension cells with cryptogein, with a fluorescence increase mimicking NO release from cells. However, HPLC separation shows that fluorescence outside cells does not at all originate from DAF-2T, the product of DAF-2 and NO, but from other yet unidentified compounds. Inside cells, other DAF molecules are formed but only a minor part is DAF-2T. The chemical nature of the novel DAF derivatives still needs to be determined. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 27% |
Student > Ph. D. Student | 2 | 18% |
Student > Bachelor | 1 | 9% |
Student > Doctoral Student | 1 | 9% |
Student > Master | 1 | 9% |
Other | 0 | 0% |
Unknown | 3 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 36% |
Agricultural and Biological Sciences | 3 | 27% |
Chemistry | 1 | 9% |
Unknown | 3 | 27% |