↓ Skip to main content
What is this page? Embed badge

Plant Functional Genomics

Overview of attention for book
Cover of 'Plant Functional Genomics'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Epigenome Profiling of Specific Plant Cell Types Using a Streamlined INTACT Protocol and ChIP-seq.
  3. Altmetric Badge
    Chapter 2 Whole-genome DNA methylation profiling with nucleotide resolution.
  4. Altmetric Badge
    Chapter 3 High-Throughput Nuclease-Mediated Probing of RNA Secondary Structure in Plant Transcriptomes
  5. Altmetric Badge
    Chapter 4 Genome-Wide Mapping of DNase I Hypersensitive Sites in Plants.
  6. Altmetric Badge
    Chapter 5 Characterization of In Vivo DNA-Binding Events of Plant Transcription Factors by ChIP-seq: Experimental Protocol and Computational Analysis.
  7. Altmetric Badge
    Chapter 6 Identification of Direct Targets of Plant Transcription Factors Using the GR Fusion Technique.
  8. Altmetric Badge
    Chapter 7 Ribosome profiling: a tool for quantitative evaluation of dynamics in mRNA translation.
  9. Altmetric Badge
    Chapter 8 Tissue-Specific Gene Expression Profiling by Cell Sorting
  10. Altmetric Badge
    Chapter 9 Translating Ribosome Affinity Purification (TRAP) Followed by RNA Sequencing Technology (TRAP-SEQ) for Quantitative Assessment of Plant Translatomes.
  11. Altmetric Badge
    Chapter 10 Rapid Immunopurification of Ribonucleoprotein Complexes of Plants
  12. Altmetric Badge
    Chapter 11 Metabolomic profiling of plant tissues.
  13. Altmetric Badge
    Chapter 12 Targeted Plant Genome Editing via the CRISPR/Cas9 Technology.
  14. Altmetric Badge
    Chapter 13 QTL Mapping Using High-Throughput Sequencing
  15. Altmetric Badge
    Chapter 14 Quantitating Plant MicroRNA-Mediated Target Repression Using a Dual-Luciferase Transient Expression System.
  16. Altmetric Badge
    Chapter 15 Persistent virus-induced gene silencing in asymptomatic accessions of Arabidopsis.
  17. Altmetric Badge
    Chapter 16 A User’s Guide to the Arabidopsis T-DNA Insertion Mutant Collections
  18. Altmetric Badge
    Chapter 17 Genome-Wide Association Mapping in Plants Exemplified for Root Growth in Arabidopsis thaliana
  19. Altmetric Badge
    Chapter 18 Tilling by sequencing.
  20. Altmetric Badge
    Chapter 19 SHOREmap v3.0: Fast and Accurate Identification of Causal Mutations from Forward Genetic Screens
  21. Altmetric Badge
    Chapter 20 Software-Assisted Stacking of Gene Modules Using GoldenBraid 2.0 DNA-Assembly Framework.
  22. Altmetric Badge
    Chapter 21 Ligation-Independent Cloning for Plant Research
  23. Altmetric Badge
    Chapter 22 Gene Functional Analysis Using Protoplast Transient Assays
  24. Altmetric Badge
    Chapter 23 Descriptive vs. Mechanistic Network Models in Plant Development in the Post-Genomic Era.
  25. Altmetric Badge
    Chapter 24 Analysis and visualization of RNA-Seq expression data using RStudio, Bioconductor, and Integrated Genome Browser
  26. Altmetric Badge
    Chapter 25 Constructing simple biological networks for understanding complex high-throughput data in plants.
Attention for Chapter 20: Software-Assisted Stacking of Gene Modules Using GoldenBraid 2.0 DNA-Assembly Framework.
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
1 X user

Citations

dimensions_citation
20 Dimensions

Readers on

mendeley
26 Mendeley
Summary X Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Software-Assisted Stacking of Gene Modules Using GoldenBraid 2.0 DNA-Assembly Framework.
Chapter number 20
Book title
Plant Functional Genomics
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2444-8_20
Pubmed ID
25757784
Book ISBNs
978-1-4939-2443-1, 978-1-4939-2444-8
Authors

Marta Vazquez-Vilar, Alejandro Sarrion-Perdigones, Peio Ziarsolo, Jose Blanca, Antonio Granell, Diego Orzaez, Vazquez-Vilar, Marta, Sarrion-Perdigones, Alejandro, Ziarsolo, Peio, Blanca, Jose, Granell, Antonio, Orzaez, Diego

Abstract

GoldenBraid (GB) is a modular DNA assembly technology for plant multigene engineering based on type IIS restriction enzymes. GB speeds up the assembly of transcriptional units from standard genetic parts and facilitates the stacking of several genes within the same T-DNA in few days. GBcloning is software-assisted with a set of online tools. The GBDomesticator tool assists in the adaptation of DNA parts to the GBstandard. The combination of GB-adapted parts to build new transcriptional units is assisted by the GB TU Assembler tool. Finally, the assembly of multigene modules is simulated by the GB Binary Assembler. All the software tools are available at www.gbcloning.org . Here, we describe in detail the assembly methodology to create a multigene construct with three transcriptional units for polyphenol metabolic engineering in plants.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 27%
Researcher 5 19%
Student > Postgraduate 3 12%
Student > Ph. D. Student 2 8%
Professor 2 8%
Other 2 8%
Unknown 5 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 46%
Biochemistry, Genetics and Molecular Biology 8 31%
Engineering 1 4%
Unknown 5 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 December 2015.
All research outputs
#18,402,666
of 22,794,367 outputs
Outputs from Methods in molecular biology
#7,903
of 13,110 outputs
Outputs of similar age
#255,771
of 353,053 outputs
Outputs of similar age from Methods in molecular biology
#479
of 996 outputs
Altmetric has tracked 22,794,367 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,110 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,053 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.