| Chapter title |
Biochemistry of Drebrin and Its Binding to Actin Filaments
|
|---|---|
| Chapter number | 3 |
| Book title |
Drebrin
|
| Published in |
Advances in experimental medicine and biology, January 2017
|
| DOI | 10.1007/978-4-431-56550-5_3 |
| Pubmed ID | |
| Book ISBNs |
978-4-43-156548-2, 978-4-43-156550-5
|
| Authors |
Ryoki Ishikawa, Ishikawa, Ryoki |
| Abstract |
Drebrin is an actin-binding protein mainly expressed in developing neurons and dendritic spine in mature neurons. To understand the functions of drebrin in vivo, we must understand its molecular properties. In this chapter, I will focus on the purification and characterization of drebrin in vitro. Drebrin binds to F-actin with a stoichiometry of 1:5~6 with a K d of 1~3 × 10(-7) M and strongly inhibits the binding of other actin-binding proteins such as tropomyosin, caldesmon, fascin, α-actinin, and cofilin. It also inhibits the activities of myosin-II and myosin-V. These results are discussed in terms of the possible roles of drebrin in the stability, dynamics, and organizations of actin structures in neuronal cells. |
Mendeley readers
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|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 38% |
| Researcher | 2 | 25% |
| Other | 1 | 13% |
| Librarian | 1 | 13% |
| Professor > Associate Professor | 1 | 13% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Medicine and Dentistry | 2 | 25% |
| Nursing and Health Professions | 1 | 13% |
| Agricultural and Biological Sciences | 1 | 13% |
| Neuroscience | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 1 | 13% |