Chapter title |
Studying Chemoattractant Signal Transduction Dynamics in Dictyostelium by BRET
|
---|---|
Chapter number | 5 |
Book title |
Chemotaxis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3480-5_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3478-2, 978-1-4939-3480-5
|
Authors |
A. F. M. Tariqul Islam, Branden M. Stepanski, Pascale G. Charest, Islam, A. F. M. Tariqul, Stepanski, Branden M., Charest, Pascale G. |
Abstract |
Understanding the dynamics of chemoattractant signaling is key to our understanding of the mechanisms underlying the directed migration of cells, including that of neutrophils to sites of infections and of cancer cells during metastasis. A model frequently used for deciphering chemoattractant signal transduction is the social amoeba Dictyostelium discoideum. However, the methods available to quantitatively measure chemotactic signaling are limited. Here, we describe a protocol to quantitatively study chemoattractant signal transduction in Dictyostelium by monitoring protein-protein interactions and conformational changes using Bioluminescence Resonance Energy Transfer (BRET). |
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