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Chapter title |
Identification of Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis
|
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Chapter number | 10 |
Book title |
Chemotaxis
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3480-5_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3478-2, 978-1-4939-3480-5
|
Authors |
Xiumei Cao, Jianshe Yan, Cao, Xiumei, Yan, Jianshe |
Abstract |
Protein-protein interactions play central roles in intercellular and intracellular signal transduction. Impairment of protein-protein interactions causes many diseases such as cancer, cardiomyopathies, diabetes, microbial infections, and genetic and neurodegenerative disorders. Immunoprecipitation is a technique in which a target protein of interest bound by an antibody is used to pull down the protein complex out of cell lysates, which can be identified by mass spectrometry. Here, we describe the protocol to immunoprecipitate and identify the components of the protein complexes of ElmoE in Dictyostelium discoideum cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 33% |
Student > Bachelor | 1 | 17% |
Student > Ph. D. Student | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Agricultural and Biological Sciences | 1 | 17% |
Medicine and Dentistry | 1 | 17% |
Unknown | 2 | 33% |