Chapter title |
Antibody-Mediated Pathogen Resistance in Plants
|
---|---|
Chapter number | 19 |
Book title |
Recombinant Proteins from Plants
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3289-4_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3288-7, 978-1-4939-3289-4
|
Authors |
Dieter Peschen, Stefan Schillberg, Rainer Fischer |
Abstract |
The methods described in this chapter were developed in order to produce transgenic plants expressing pathogen-specific single-chain variable fragment (scFv) antibodies fused to antifungal peptides (AFPs), conferring resistance against fungal pathogens. We describe the selection from a phage display library of avian scFv antibodies that recognize cell surface proteins on fungi from the genus Fusarium, and the construction of scFv-AFP fusion protein constructs followed by their transient expression in tobacco (Nicotiana spp.) plants and stable expression in Arabidopsis thaliana plants. Using these techniques, the antibody fusion with the most promising in vitro activity can be used to generate transgenic plants that are resistant to pathogens such as Fusarium oxysporum f. sp. matthiolae. |
Mendeley readers
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Demographic breakdown
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Researcher | 2 | 22% |
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Unknown | 4 | 44% |
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