Chapter title |
Transient Protein Expression by Agroinfiltration in Lettuce
|
---|---|
Chapter number | 4 |
Book title |
Recombinant Proteins from Plants
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3289-4_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3288-7, 978-1-4939-3289-4
|
Authors |
Qiang Chen, Matthew Dent, Jonathan Hurtado, Jake Stahnke, Alyssa McNulty, Kahlin Leuzinger, Huafang Lai |
Abstract |
Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 2% |
New Zealand | 1 | 2% |
Unknown | 45 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 26% |
Student > Master | 6 | 13% |
Researcher | 6 | 13% |
Student > Doctoral Student | 3 | 6% |
Unspecified | 3 | 6% |
Other | 6 | 13% |
Unknown | 11 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 12 | 26% |
Biochemistry, Genetics and Molecular Biology | 11 | 23% |
Unspecified | 3 | 6% |
Chemical Engineering | 3 | 6% |
Engineering | 3 | 6% |
Other | 3 | 6% |
Unknown | 12 | 26% |