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Small Non-Coding RNAs

Overview of attention for book
Cover of 'Small Non-Coding RNAs'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Small Non-Coding RNAs: A Quick Look in the Rearview Mirror.
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    Chapter 2 Alcoholic Precipitation of Small Non-Coding RNAs
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    Chapter 3 Quantification and Quality Control of a Small Non-Coding RNA Preparation
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    Chapter 4 Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting
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    Chapter 5 Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes
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    Chapter 6 Direct Cloning of Double-Stranded RNAs
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    Chapter 7 Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation.
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    Chapter 8 Fluorescence In Situ Hybridization of Small Non-Coding RNAs
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    Chapter 9 RT-qPCR-Based Quantification of Small Non-Coding RNAs.
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    Chapter 10 Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs.
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    Chapter 11 miR-RACE: An Effective Approach to Accurately Determine the Sequence of Computationally Identified miRNAs.
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    Chapter 12 Probing Small Non-Coding RNAs Structures
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    Chapter 13 cDNA Library Generation for the Analysis of Small RNAs by High-Throughput Sequencing.
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    Chapter 14 CLIP-Seq to Discover Transcriptome-Wide Imprinting of RNA Binding Proteins in Living Cells.
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    Chapter 15 Microarray Analysis of Small Non-Coding RNAs
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    Chapter 16 RLM-RACE, PPM-RACE, and qRT-PCR: An Integrated Strategy to Accurately Validate miRNA Target Genes.
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    Chapter 17 Dual Luciferase Gene Reporter Assays to Study miRNA Function.
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    Chapter 18 Gene Expression Knockdown by Transfection of siRNAs into Mammalian Cells
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    Chapter 19 Efficient and Selective Knockdown of Small Non-Coding RNAs.
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    Chapter 20 Cell-SELEX: In Vitro Selection of Synthetic Small Specific Ligands.
  22. Altmetric Badge
    Chapter 21 Small Non-Coding RNAs and Aptamers in Diagnostics and Therapeutics.
Attention for Chapter 13: cDNA Library Generation for the Analysis of Small RNAs by High-Throughput Sequencing.
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  • Good Attention Score compared to outputs of the same age and source (66th percentile)

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Chapter title
cDNA Library Generation for the Analysis of Small RNAs by High-Throughput Sequencing.
Chapter number 13
Book title
Small Non-Coding RNAs
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2547-6_13
Pubmed ID
25791597
Book ISBNs
978-1-4939-2546-9, 978-1-4939-2547-6
Authors

Jennifer Gebetsberger, Roger Fricker, Norbert Polacek, Gebetsberger J, Fricker R, Polacek N, Gebetsberger, Jennifer, Fricker, Roger, Polacek, Norbert

Abstract

The RNome of a cell is highly diverse and consists besides messenger RNAs (mRNAs), transfer RNAs (tRNAs), and ribosomal RNAs (rRNAs) also of other small and long transcript entities without apparent coding potential. This class of molecules, commonly referred to as non-protein-coding RNAs (ncRNAs), is involved in regulating numerous biological processes and thought to contribute to cellular complexity. Therefore, much effort is put into their identification and further functional characterization. Here we provide a cost-effective and reliable method for cDNA library construction of small RNAs in the size range of 20-500 residues. The effectiveness of the described method is demonstrated by the analysis of ribosome-associated small RNAs in the eukaryotic model organism Trypanosoma brucei.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 30%
Student > Bachelor 2 20%
Student > Ph. D. Student 1 10%
Unspecified 1 10%
Other 1 10%
Other 0 0%
Unknown 2 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 40%
Unspecified 1 10%
Agricultural and Biological Sciences 1 10%
Medicine and Dentistry 1 10%
Engineering 1 10%
Other 0 0%
Unknown 2 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 December 2015.
All research outputs
#14,806,069
of 22,796,179 outputs
Outputs from Methods in molecular biology
#4,682
of 13,111 outputs
Outputs of similar age
#197,949
of 353,053 outputs
Outputs of similar age from Methods in molecular biology
#295
of 996 outputs
Altmetric has tracked 22,796,179 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,111 research outputs from this source. They receive a mean Attention Score of 3.3. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,053 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.

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