| Chapter title |
Probing Cancer Cell Metabolism Using NMR Spectroscopy
|
|---|---|
| Chapter number | 6 |
| Book title |
Tumor Microenvironment
|
| Published in |
Advances in experimental medicine and biology, January 2016
|
| DOI | 10.1007/978-3-319-26666-4_6 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-926664-0, 978-3-31-926666-4
|
| Authors |
Kate E. R. Hollinshead, Debbie S. Williams, Daniel A. Tennant, Christian Ludwig, Hollinshead, Kate E. R., Williams, Debbie S., Tennant, Daniel A., Ludwig, Christian |
| Abstract |
Altered cellular metabolism is now accepted to be at the core of many diseases including cancer. Over the past 20 years, NMR has become a core technology to study these metabolic perturbations in detail. This chapter reviews current NMR-based methods for steady-state metabolism and, in particular, the use of non-radioactive stable isotope-enriched tracers. Opportunities and challenges for each method, such as 1D (1)H NMR spectroscopy and (13)C carbon-based NMR spectroscopic methods, are discussed. Ultimately, the combination of NMR and mass spectra as orthogonal technologies are required to compensate for the drawbacks of each technique when used singly are discussed. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 36% |
| Student > Doctoral Student | 1 | 9% |
| Student > Bachelor | 1 | 9% |
| Student > Master | 1 | 9% |
| Student > Ph. D. Student | 1 | 9% |
| Other | 2 | 18% |
| Unknown | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 27% |
| Agricultural and Biological Sciences | 3 | 27% |
| Chemistry | 1 | 9% |
| Engineering | 1 | 9% |
| Unknown | 3 | 27% |