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Handbook for Rhizobia

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Cover of 'Handbook for Rhizobia'

Table of Contents

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    Book Overview
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    Chapter 1 Collecting Nodules and Isolating Rhizobia
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    Chapter 2 Observing the Infection Process
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    Chapter 3 Cultural Properties, Cell Morphology, and Nutritional Requirements of Rhizobia
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    Chapter 4 Demonstrating Genetic Diversity in Rhizobia Using Patterns of Carbohydrate Utilization and Intrinsic Antibiotic Resistance
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    Chapter 5 Quantifying the Growth of Rhizobia
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    Chapter 6 Counting Rhizobia by a Plant Infection Method
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    Chapter 7 Counting Serologically Specific Rhizobia in Soil and Peat Inoculants Using Membrane Filters and Immunofluorescence
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    Chapter 8 Developing Antisera
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    Chapter 9 Somatic Agglutination Reactions with Pure Cultures of Rhizobia
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    Chapter 10 Agglutinating Antigens from Root Nodules
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    Chapter 11 Performing Rhizobial Antigen-Antibody Reactions by Gel Immunodiffusion
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    Chapter 12 Determining Strain Occupancy in Soybean Nodules by Gel Immunodiffusion
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    Chapter 13 Producing and Applying Fluorescent Antibodies
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    Chapter 14 Identifying Rhizobia by the Indirect Enzyme-Linked Immunosorbent Assay
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    Chapter 15 Identifying Rhizobia by Immunoblot
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    Chapter 16 Isolating Spontaneous Antibiotic-Resistant Mutants of Rhizobia
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    Chapter 17 Analyzing Nodule Occupancy Using Antibiotic-Resistant Markers
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    Chapter 18 Distinguishing between Strains of Rhizobia by Rhizobiophage Susceptibility
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    Chapter 19 Testing For Genetic Compatibility between Rhizobia and Legumes
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    Chapter 20 Screening Rhizobia for Nitrogen-Fixation Potential
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    Chapter 21 Screening Effective Strains of Rhizobia in Potted Field Soil
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    Chapter 22 Verifying the Nitrogen-Fixing Potential of Glasshouse-Selected Soybean Rhizobia in the Field Environment
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    Chapter 23 Evaluating the Symbiotic Potential of Indigenous Rhizobial Populations of Soils Using the Whole-Soil Inocula Technique
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    Chapter 24 Investigating the Importance of Optimal Soil Fertility in the Response of a Legume to Inoculation with Rhizobia
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    Chapter 25 Producing Broth Cultures in Simple Glass Fermentors
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    Chapter 26 Producing Inoculum in a Steel Fermentor
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    Chapter 27 Preparing a Range of Carrier Materials and Producing Inoculants
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    Chapter 28 Preparing Inoculants Using Diluted Cultures of Rhizobia and Presterilized Peat
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    Chapter 29 Testing the Survival of Rhizobia on Inoculated Seeds
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    Chapter 30 Analyzing Plasmid Profiles of Rhizobium spp. by a Modified Eckhardt Vertical Gel Electrophoresis Procedure
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    Chapter 31 Isolating and Purifying Genomic DNA of Rhizobia Using a Large-Scale Method
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    Chapter 32 Isolating and Purifying Genomic DNA of Rhizobia Using a Rapid Small-Scale Method
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    Chapter 33 Digesting Genomic DNA of Rhizobia with Restriction Endonucleases
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    Chapter 34 Separating Restriction Fragments of Genomic DNA by Horizontal Agarose Gel Electrophoresis
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    Chapter 35 Transferring Electrophoretically Separated DNA from Agarose Gels to a Membrane by Southern Blotting
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    Chapter 36 Preparing a DNA Probe for Detecting the nif Genes on Symbiotic Plasmids of Rhizobium spp.
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    Chapter 37 Incorporating a Nonradioactive Label into a DNA Probe by Nick Translation
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    Chapter 38 Using a Nonradioactively Labeled nif KDH Gene Probe to Locate Complementary Sequences of Rhizobial DNA Immobilized on Membranes
Attention for Chapter 38: Using a Nonradioactively Labeled nif KDH Gene Probe to Locate Complementary Sequences of Rhizobial DNA Immobilized on Membranes
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Chapter title
Using a Nonradioactively Labeled nif KDH Gene Probe to Locate Complementary Sequences of Rhizobial DNA Immobilized on Membranes
Chapter number 38
Book title
Handbook for Rhizobia
Published by
Springer, New York, NY, January 1994
DOI 10.1007/978-1-4613-8375-8_38
Book ISBNs
978-1-4613-8377-2, 978-1-4613-8375-8
Authors

Padma Somasegaran, Heinz J. Hoben, Somasegaran, Padma, Hoben, Heinz J.