Handbook for Rhizobia

Table of Contents

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   Book Overview
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   Chapter 1 Collecting Nodules and Isolating Rhizobia
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   Chapter 2 Observing the Infection Process
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   Chapter 3 Cultural Properties, Cell Morphology, and Nutritional Requirements of Rhizobia
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   Chapter 4 Demonstrating Genetic Diversity in Rhizobia Using Patterns of Carbohydrate Utilization and Intrinsic Antibiotic Resistance
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   Chapter 5 Quantifying the Growth of Rhizobia
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   Chapter 6 Counting Rhizobia by a Plant Infection Method
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   Chapter 7 Counting Serologically Specific Rhizobia in Soil and Peat Inoculants Using Membrane Filters and Immunofluorescence
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   Chapter 8 Developing Antisera
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    Chapter 9 Somatic Agglutination Reactions with Pure Cultures of Rhizobia
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    Chapter 10 Agglutinating Antigens from Root Nodules
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    Chapter 11 Performing Rhizobial Antigen-Antibody Reactions by Gel Immunodiffusion
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    Chapter 12 Determining Strain Occupancy in Soybean Nodules by Gel Immunodiffusion
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    Chapter 13 Producing and Applying Fluorescent Antibodies
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    Chapter 14 Identifying Rhizobia by the Indirect Enzyme-Linked Immunosorbent Assay
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    Chapter 15 Identifying Rhizobia by Immunoblot
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    Chapter 16 Isolating Spontaneous Antibiotic-Resistant Mutants of Rhizobia
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    Chapter 17 Analyzing Nodule Occupancy Using Antibiotic-Resistant Markers
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    Chapter 18 Distinguishing between Strains of Rhizobia by Rhizobiophage Susceptibility
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    Chapter 19 Testing For Genetic Compatibility between Rhizobia and Legumes
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    Chapter 20 Screening Rhizobia for Nitrogen-Fixation Potential
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    Chapter 21 Screening Effective Strains of Rhizobia in Potted Field Soil
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    Chapter 22 Verifying the Nitrogen-Fixing Potential of Glasshouse-Selected Soybean Rhizobia in the Field Environment
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    Chapter 23 Evaluating the Symbiotic Potential of Indigenous Rhizobial Populations of Soils Using the Whole-Soil Inocula Technique
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    Chapter 24 Investigating the Importance of Optimal Soil Fertility in the Response of a Legume to Inoculation with Rhizobia
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    Chapter 25 Producing Broth Cultures in Simple Glass Fermentors
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    Chapter 26 Producing Inoculum in a Steel Fermentor
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    Chapter 27 Preparing a Range of Carrier Materials and Producing Inoculants
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    Chapter 28 Preparing Inoculants Using Diluted Cultures of Rhizobia and Presterilized Peat
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    Chapter 29 Testing the Survival of Rhizobia on Inoculated Seeds
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    Chapter 30 Analyzing Plasmid Profiles of Rhizobium spp. by a Modified Eckhardt Vertical Gel Electrophoresis Procedure
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    Chapter 31 Isolating and Purifying Genomic DNA of Rhizobia Using a Large-Scale Method
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    Chapter 32 Isolating and Purifying Genomic DNA of Rhizobia Using a Rapid Small-Scale Method
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    Chapter 33 Digesting Genomic DNA of Rhizobia with Restriction Endonucleases
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    Chapter 34 Separating Restriction Fragments of Genomic DNA by Horizontal Agarose Gel Electrophoresis
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    Chapter 35 Transferring Electrophoretically Separated DNA from Agarose Gels to a Membrane by Southern Blotting
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    Chapter 36 Preparing a DNA Probe for Detecting the nif Genes on Symbiotic Plasmids of Rhizobium spp.
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    Chapter 37 Incorporating a Nonradioactive Label into a DNA Probe by Nick Translation
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    Chapter 38 Using a Nonradioactively Labeled nif KDH Gene Probe to Locate Complementary Sequences of Rhizobial DNA Immobilized on Membranes