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Mendeley readers
| Chapter title |
Visualizing Virus-Derived dsRNA Using Antibody-Independent and -Dependent Methods
|
|---|---|
| Chapter number | 5 |
| Book title |
Innate Antiviral Immunity
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7237-1_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7236-4, 978-1-4939-7237-1
|
| Authors |
Sarah J. Poynter, Stephanie J. DeWitte-Orr, Poynter, Sarah J., DeWitte-Orr, Stephanie J. |
| Abstract |
Long double-stranded (ds) RNA molecules are produced as a byproduct of viral replication. Studying virus-derived dsRNA is important for understanding virus replication, understanding host responses to virus infections, and as a diagnostic tool for virus presence and replication. Here, we describe four different techniques for visualizing dsRNA; two antibody-dependent methods (immunoblotting and immunocytochemistry), as well as two antibody-independent methods (differential digestion and acridine orange staining). The benefits and disadvantages of each technique are also discussed. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 29% |
| Other | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Unknown | 3 | 43% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Nursing and Health Professions | 1 | 14% |
| Immunology and Microbiology | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
| Engineering | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 2 | 29% |