Chapter title |
Isolation, Purification, and Culture of Primary Murine Sensory Neurons
|
---|---|
Chapter number | 15 |
Book title |
Innate Antiviral Immunity
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7237-1_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7236-4, 978-1-4939-7237-1
|
Authors |
Sarah Katzenell, Jorge R. Cabrera, Brian J. North, David A. Leib, Katzenell, Sarah, Cabrera, Jorge R., North, Brian J., Leib, David A. |
Abstract |
Cultured primary neurons have been of extraordinary value for the study of neuronal anatomy, cell biology, and physiology. While use of neuronal cell lines has ease and utility, there are often caveats that arise due to their mitotic nature. This methods article presents detailed methodology for the preparation, purification, and culture of adult murine sensory neurons for the study of herpes simplex virus lytic and latent infections. While virology is the application for our laboratory, these cultures also have broad utility for neurobiologists and cell biologists. While these primary cultures have been highly informative, the methodology is challenging to many investigators. Through publication of this highly detailed protocol, it is our hope that the use of this culture system can spread in the field to allow more rapid progress in furthering our understanding of neurotropic virus infection. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 56 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 18 | 32% |
Researcher | 8 | 14% |
Student > Postgraduate | 4 | 7% |
Student > Master | 4 | 7% |
Student > Bachelor | 3 | 5% |
Other | 6 | 11% |
Unknown | 13 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 15 | 27% |
Agricultural and Biological Sciences | 9 | 16% |
Neuroscience | 5 | 9% |
Immunology and Microbiology | 4 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 7% |
Other | 5 | 9% |
Unknown | 14 | 25% |