Chapter title |
Psychoneuroimmunology and natural killer cells: the chromium release whole blood assay.
|
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Chapter number | 16 |
Book title |
Psychoneuroimmunology
|
Published in |
Methods in molecular biology, August 2012
|
DOI | 10.1007/978-1-62703-071-7_16 |
Pubmed ID | |
Book ISBNs |
978-1-62703-070-0, 978-1-62703-071-7
|
Authors |
Mary Ann Fletcher, Zachary Barnes, Gordon Broderick, Nancy G. Klimas, Fletcher, Mary Ann, Barnes, Zachary, Broderick, Gordon, Klimas, Nancy G. |
Abstract |
Natural killer (NK) cells are an essential component of innate immunity. These lymphocytes are also sensitive barometers of the effects of endogenous and exogenous stressors on the immune system. This chapter will describe a chromium ((51)Cr) release bioassay designed to measure the target cell killing capacity of NK cells (NKCC). Key features of the cytotoxicity assay are that it is done with whole blood and that numbers of effector cells are determined for each sample by flow cytometry and lymphocyte count. Effector cells are defined as CD3-CD56+ lymphocytes. Target cells are the K562 eyrthroleukemia cell line. Killing capacity is defined as number of target cells killed per effector cell, at an effector cell/target cell ratio of 1:1 during a 4 h in vitro assay. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
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Germany | 1 | 17% |
Unknown | 5 | 83% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 33% |
Professor | 1 | 17% |
Student > Ph. D. Student | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 4 | 67% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Unknown | 1 | 17% |