Chapter title |
Förster resonance energy transfer-based imaging at the cell surface of live cells.
|
---|---|
Chapter number | 16 |
Book title |
Ion Channels
|
Published in |
Methods in molecular biology, March 2013
|
DOI | 10.1007/978-1-62703-351-0_16 |
Pubmed ID | |
Book ISBNs |
978-1-62703-350-3, 978-1-62703-351-0
|
Authors |
Bierbower SM, Shapiro MS, Sonya M. Bierbower, Mark S. Shapiro, Bierbower, Sonya M., Shapiro, Mark S. |
Abstract |
Understanding the molecular mechanisms of protein-protein interactions at the cell surface of living cells is fundamental to identifying the nature of cellular processes. Here, we discuss how fluorescence-based approaches have been successfully developed to visualize protein-protein interactions in living cells. Förster resonance energy transfer (FRET) is unique in generating fluorescence signals between proteins that are highly spatially sensitive. Furthermore, total internal reflectance fluorescence (TIRF) microscopy combined with FRET is a robust technique used to assay protein/protein interactions and the functionality of proteins assembled at the cell surface membrane. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 69 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 16 | 23% |
Researcher | 12 | 17% |
Student > Master | 12 | 17% |
Student > Bachelor | 6 | 9% |
Student > Postgraduate | 4 | 6% |
Other | 13 | 19% |
Unknown | 6 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 18 | 26% |
Biochemistry, Genetics and Molecular Biology | 14 | 20% |
Neuroscience | 12 | 17% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 6% |
Chemistry | 3 | 4% |
Other | 6 | 9% |
Unknown | 12 | 17% |