| Chapter title |
Optical Control of Ligand-Gated Ion Channels
|
|---|---|
| Chapter number | 32 |
| Book title |
Ion Channels
|
| Published in |
Methods in molecular biology, January 2013
|
| DOI | 10.1007/978-1-62703-351-0_32 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-350-3, 978-1-62703-351-0
|
| Authors |
Stephanie Szobota, Catherine McKenzie, Harald Janovjak, Szobota, Stephanie, McKenzie, Catherine, Janovjak, Harald |
| Abstract |
In the vibrant field of optogenetics, optics and genetic targeting are combined to commandeer cellular functions, such as the neuronal action potential, by optically stimulating light-sensitive ion channels expressed in the cell membrane. One broadly applicable manifestation of this approach are covalently attached photochromic tethered ligands (PTLs) that allow activating ligand-gated ion channels with outstanding spatial and temporal resolution. Here, we describe all steps towards the successful development and application of PTL-gated ion channels in cell lines and primary cells. The basis for these experiments forms a combination of molecular modeling, genetic engineering, cell culture, and electrophysiology. The light-gated glutamate receptor (LiGluR), which consists of the PTL-functionalized GluK2 receptor, serves as a model. |
X Demographics
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|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 17% |
| Unknown | 5 | 83% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 33% |
| Professor | 1 | 17% |
| Student > Bachelor | 1 | 17% |
| Researcher | 1 | 17% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Neuroscience | 1 | 17% |
| Unknown | 2 | 33% |