Chapter title |
Nucleic Acid Crystallography
|
---|---|
Chapter number | 22 |
Book title |
Nucleic Acid Crystallography
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2763-0_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2762-3, 978-1-4939-2763-0
|
Authors |
D'Ascenzo, Luigi, Auffinger, Pascal, Luigi D’Ascenzo, Pascal Auffinger |
Abstract |
Nucleic acid crystallization buffers contain a large variety of chemicals fitting specific needs. Among them, anions are often solely considered for pH-regulating purposes and as cationic co-salts while their ability to directly bind to nucleic acid structures is rarely taken into account. Here we review current knowledge related to the use of anions in crystallization buffers along with data on their biological prevalence. Chloride ions are frequently identified in crystal structures but display low cytosolic concentrations. Hence, they are thought to be distant from nucleic acid structures in the cell. Sulfate ions are also frequently identified in crystal structures but their localization in the cell remains elusive. Nevertheless, the characterization of the binding properties of these ions is essential for better interpreting the solvent structure in crystals and consequently, avoiding mislabeling of electron densities. Furthermore, understanding the binding properties of these anions should help to get clues related to their potential effects in crowded cellular environments. |
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