Chapter title |
Advanced Crystallographic Data Collection Protocols for Experimental Phasing
|
---|---|
Chapter number | 11 |
Book title |
Nucleic Acid Crystallography
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2763-0_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2762-3, 978-1-4939-2763-0
|
Authors |
Aaron D. Finke, Ezequiel Panepucci, Clemens Vonrhein, Meitian Wang, Gérard Bricogne, Vincent Oliéric, Finke, Aaron D., Panepucci, Ezequiel, Vonrhein, Clemens, Wang, Meitian, Bricogne, Gérard, Oliéric, Vincent |
Abstract |
Experimental phasing by single- or multi-wavelength anomalous dispersion (SAD or MAD) has become the most popular method of de novo macromolecular structure determination. Continuous advances at third-generation synchrotron sources have enabled the deployment of rapid data collection protocols that are capable of recording SAD or MAD data sets. However, procedural simplifications driven by the pursuit of high throughput have led to a loss of sophistication in data collection strategies, adversely affecting measurement accuracy from the viewpoint of anomalous phasing. In this chapter, we detail optimized strategies for collecting high-quality data for experimental phasing, with particular emphasis on minimizing errors from radiation damage as well as from the instrument. This chapter also emphasizes data processing for "on-the-fly" decision-making during data collection, a critical process when data quality depends directly on information gathered while at the synchrotron. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 6 | 50% |
Student > Ph. D. Student | 3 | 25% |
Unspecified | 1 | 8% |
Student > Postgraduate | 1 | 8% |
Unknown | 1 | 8% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 7 | 58% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Unspecified | 1 | 8% |
Chemistry | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Other | 0 | 0% |
Unknown | 1 | 8% |