| Chapter title |
Novel Biomarkers in the Continuum of Breast Cancer
|
|---|---|
| Chapter number | 11 |
| Book title |
Novel Biomarkers in the Continuum of Breast Cancer
|
| Published in |
Advances in experimental medicine and biology, January 2016
|
| DOI | 10.1007/978-3-319-22909-6_11 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-922908-9, 978-3-31-922909-6
|
| Authors |
Parsons, Heather A, Beaver, Julia A, Park, Ben H, Heather A. Parsons, Julia A. Beaver, Ben H. Park |
| Abstract |
Circulating cell-free DNA (ccfDNA)-first identified in 1947-is "naked" DNA that is free-floating in the blood, and derived from both normal and diseased cells. In the 1970s, scientists observed that patients with cancer had elevated levels of ccfDNA as compared to their healthy, cancer-free counterparts. The maternal fetal medicine community first developed techniques to identify the small fraction of fetal-derived ccfDNA for diagnostic purposes. Similarly, due to the presence of tumor-specific (somatic) variations in all cancers, the fraction of circulating cell-free plasma tumor DNA (ptDNA) in the larger pool of ccfDNA derived from normal cells can serve as extremely specific blood-based biomarkers for a patient's cancer. In theory this "liquid biopsy" can provide a real-time assessment of molecular tumor genotype (qualitative) and existing tumor burden (quantitative). Historically, the major limitation for ptDNA as a biomarker has been related to a low detection rate; however, current and developing techniques have improved sensitivity dramatically. In this chapter, we discuss these methods, including digital polymerase chain reaction and various approaches to tagged next-generation sequencing. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 50% |
| Sweden | 1 | 25% |
| Brazil | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 1 | 2% |
| Ireland | 1 | 2% |
| Unknown | 53 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 20% |
| Student > Ph. D. Student | 9 | 16% |
| Other | 7 | 13% |
| Student > Bachelor | 4 | 7% |
| Student > Doctoral Student | 4 | 7% |
| Other | 9 | 16% |
| Unknown | 11 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 22 | 40% |
| Biochemistry, Genetics and Molecular Biology | 9 | 16% |
| Agricultural and Biological Sciences | 9 | 16% |
| Chemistry | 2 | 4% |
| Neuroscience | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 11 | 20% |