Chapter 1 Protein Engineering: Past, Present, and Future

Chapter 2 Rational and Semirational Protein Design

Chapter 3 Computational Analysis of Protein Tunnels and Channels

Chapter 4 YASARA: A Tool to Obtain Structural Guidance in Biocatalytic Investigations

Chapter 5 A Computational Library Design Protocol for Rapid Improvement of Protein Stability: FRESCO

Chapter 6 Directed Evolution of Proteins Based on Mutational Scanning

Chapter 7 A Brief Guide to the High-Throughput Expression of Directed Evolution Libraries

Chapter 8 Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli Using MTP Shakers

Chapter 9 Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification

Chapter 10 Functional Analysis of Membrane Proteins Produced by Cell-Free Translation

Chapter 11 Practical Considerations Regarding the Choice of the Best High-Throughput Assay

Chapter 12 High-Throughput Screening Assays for Lipolytic Enzymes

Chapter 13 Continuous High-Throughput Colorimetric Assays for α -Transaminases

Chapter 15 Directed Coevolution of Two Cellulosic Enzymes in Escherichia coli Based on Their Synergistic Reactions

Chapter 16 Program-Guided Design of High-Throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation

Chapter 17 Solid-Phase Agar Plate Assay for Screening Amine Transaminases

Chapter 18 Ultrahigh-Throughput Screening of Single-Cell Lysates for Directed Evolution and Functional Metagenomics

Chapter 19 Isolation of pH-Sensitive Antibody Fragments by Fluorescence-Activated Cell Sorting and Yeast Surface Display

Chapter 20 Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophilus

Chapter 21 Erratum to: Functional Analysis of Membrane Proteins Produced by Cell-Free Translation