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| Chapter title |
Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophilus
|
|---|---|
| Chapter number | 20 |
| Book title |
Protein Engineering
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7366-8_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7364-4, 978-1-4939-7366-8
|
| Authors |
Jörg Claren, Thomas Schwab, Reinhard Sterner, Claren, Jörg, Schwab, Thomas, Sterner, Reinhard |
| Abstract |
The selection of optimized enzymes from gene libraries is important, both for basic and applied research. Here, we first describe the generation of plasmid-borne libraries using error-prone PCR and highly competent Escherichia coli cells. We then provide protocols for the use of these libraries for auxotrophic selection assays with E. coli and the extremely thermophilic bacterium Thermus thermophilus as hosts. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |