Angelini, Alessandro, Chen, Tiffany F, de Picciotto, Seymour, Yang, Nicole J, Tzeng, Alice, Santos, Michael S, Van Deventer, James A, Traxlmayr, Michael W, Wittrup, K Dane, Alessandro Angelini, Tiffany F. Chen, Seymour de Picciotto, Nicole J. Yang, Alice Tzeng, Michael S. Santos, James A. Van Deventer, Michael W. Traxlmayr, K. Dane Wittrup, Chen, Tiffany F., Picciotto, Seymour de, Yang, Nicole J., Santos, Michael S., Deventer, James A. Van, Traxlmayr, Michael W., Wittrup, K. Dane
Abstract
Yeast surface display is a powerful technology for engineering a broad range of protein scaffolds. This protocol describes the process for de novo isolation of protein binders from large combinatorial libraries displayed on yeast by using magnetic bead separation followed by flow cytometry-based selection. The biophysical properties of isolated single clones are subsequently characterized, and desired properties are further enhanced through successive rounds of mutagenesis and flow cytometry selections, resulting in protein binders with increased stability, affinity, and specificity for target proteins of interest.
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2021.
All research outputs
#6,411,532
of 22,780,165 outputs
Outputs from Methods in molecular biology
#1,944
of 13,094 outputs
Outputs of similar age
#87,975
of 352,944 outputs
Outputs of similar age from Methods in molecular biology
#148
of 996 outputs
Altmetric has tracked 22,780,165 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 13,094 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,944 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.
