Chapter title |
Yeast Display-Based Antibody Affinity Maturation Using Detergent-Solubilized Cell Lysates.
|
---|---|
Chapter number | 4 |
Book title |
Yeast Surface Display
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2748-7_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2747-0, 978-1-4939-2748-7
|
Authors |
Tillotson, Benjamin J, Lajoie, Jason M, Shusta, Eric V, Benjamin J. Tillotson, Jason M. Lajoie, Eric V. Shusta, Tillotson, Benjamin J., Lajoie, Jason M., Shusta, Eric V. |
Abstract |
It is often desired to identify or engineer antibodies that target membrane proteins (MPs). However, due to their inherent insolubility in aqueous solutions, MPs are often incompatible with in vitro antibody discovery and optimization platforms. Recently, we adapted yeast display technology to accommodate detergent-solubilized cell lysates as sources of MP antigens. The following protocol details the incorporation of cell lysates into a kinetic screen designed to obtain antibodies with improved affinity via slowed dissociation from an MP antigen. |
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