| Chapter title |
Measurement of PTEN by Flow Cytometry
|
|---|---|
| Chapter number | 4 |
| Book title |
PTEN
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3299-3_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3297-9, 978-1-4939-3299-3
|
| Authors |
John F. Woolley, Leonardo Salmena |
| Abstract |
Recent advancements have driven the development of smaller footprint, less expensive, and user-friendly flow cytometers introducing the technology to more users.Flow cytometry is an established tool for multiparametric analysis of various important cellular characteristics. Fluorescent dyes or fluorophore-conjugated antibodies allow for measurement of protein expression, identification of cell populations, or DNA content analysis. This is combined with analysis of light-scattering detection to determine cell size and complexity to allow for the study of complex cell samples, such as whole blood. Through antibody staining for a variety of surface markers as well as intracellular proteins we can also elucidate intracellular signaling, and phosphor-signaling, on a single-cell basis.Here we describe the application of flow cytometry analysis to the tumor suppressor PTEN in various cancer cell lines and a mouse model. |
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