Chapter title |
Humanization of the Mouse Mammary Gland
|
---|---|
Chapter number | 10 |
Book title |
Mammary Stem Cells
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2519-3_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2518-6, 978-1-4939-2519-3
|
Authors |
A. Wronski, L. M. Arendt, Charlotte Kuperwasser |
Abstract |
Although mouse models have provided invaluable information on the mechanisms of mammary gland development, anatomical and developmental differences between human and mice limit full understanding of this fundamental process. Humanization of the mouse mammary gland by injecting immortalized human breast stromal cells into the cleared murine mammary fat pad enables the growth and development of human mammary epithelial cells or tissue. This facilitates the characterization of human mammary gland development or tumorigenesis by utilizing the mouse mammary fat pad. Here we describe the process of isolating human mammary stromal and epithelial cells as well as their introduction into the mammary fat pads of immunocompromised mice. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 44% |
Professor > Associate Professor | 1 | 11% |
Student > Doctoral Student | 1 | 11% |
Unknown | 3 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
Veterinary Science and Veterinary Medicine | 1 | 11% |
Agricultural and Biological Sciences | 1 | 11% |
Engineering | 1 | 11% |
Other | 0 | 0% |
Unknown | 3 | 33% |